KMID : 0043320110340010031
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Archives of Pharmacal Research 2011 Volume.34 No. 1 p.31 ~ p.36
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Effects of psoralen from Psoralea corylifolia on quinone reductase, ornithine decarboxylase, and JB6 cells transformation promotion
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Lee Sung-Jin
Nam Kung-Woo Mar Woong-Chon
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Abstract
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The cancer chemopreventive effect of psoralen isolated from the seeds of Psoralea corylifolia was investigated in the induction of quinone reductase (QR) activity, intracellular detoxification enzyme, inhibition of 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced ornithine decarboxylase (ODC) activity, a key regulatory enzyme for polyamine metabolism, and tumor promotion in mouse epidermal JB6 cells, sensitive to tumor promoters (clone 415a P+ cells), which are related to suppress multistage carcinogenesis including initiation and promotion. Psoralen was isolated and identified from the ethyl acetate-soluble fraction of the methanolic extract from the seeds. Psoralen was active in induction of QR activity, the concentration of psoralen required to induce 1.5 fold QR activity was 14.8 ¥ìg/mL. Also, this pure compound inhibited TPA-induced ODC activity by 50% (designated IC50) at the concentration 15.6 ¥ìg/mL and exhibited inhibition of TPA-induced tumor promotion in mouse epidermal JB6 cells with an IC50 value of 17.1 ¥ìg/mL. Therefore, it is extrapolated that psoralen has the potential capable of inhibiting the initiation and/or promotion stage of carcinogenesis by induction of QR activity, inhibition of TPA-induced ODC activity and mouse epidermal JB6 cells tumor promotion.
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KEYWORD
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Psoralea corylifolia, Psoralen, Ornithine decarboxylase, Quinone reductase, JB6 cells, Cancer chemoprevention
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